Bovine Insulin Like Growth Factor Binding Protein 2

Mfr.No.

E2083Bo

Description

This sandwich kit is for the accurate quantitative detection of Bovine Insulin-like Growth Factors Binding Protein 2 (also known as IGFBP-2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IBP-2;IGF-binding protein 2;IGFBP 2;IGFBP2;IGFBP-2;Insulin-like growth factor-binding protein 2

Reactivity

Bovine

Detection Range

3-900ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

34015

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.52ng/ml

Background

Inhibits IGF-mediated growth and developmental rates (By similarity). IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Gene ID	282260
UniProt ID	P13384
Antigen	IGFBP2

Research Area

Signal Transduction; Cancer

Protocol

These standard curves of E2083Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/ml	2.194	2.238	2.187
	2.282
240ng/ml	1.692	1.619	1.568
	1.546
120ng/ml	0.976	1.069	1.019
	1.162
60ng/ml	0.686	0.663	0.612
	0.639
30ng/ml	0.494	0.452	0.401
	0.409
0ng/ml	0.051	0.05	0
	0.05

Precision

We measured random samples of E2083Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	152.4	10.62	7
Intra-Assay	2	18	308.8	19.83	6.4
Intra-Assay	3	18	57.4	3.72	6.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100453	E2083Bo	96T	$458		Add to Cart
BHE12100453	E2083Bo	48T	$320		Add to Cart

Bovine Insulin Like Growth Factor Binding Protein 2 - IGFBP2 ELISA Kit

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