Bovine Immunoglobulin A,IgA ELISA Kit

Mfr.No.

CSB-E12018B

Alternative Names

Immunoglobulin A

Reactivity

Bovine

Detection Range

0.39 ug/mL-25 ug/mL

Sample Type

Tissue homogenates, Serum, Plasma, Cell culture supernatants

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human PRSS3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-98
EDTA plasma (n=4)	97	91-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PRSS3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	92
	Range %	87-96
1:200	Average %	103
	Range %	98-107
1:400	Average %	105
	Range %	101-109
1:800	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.584	2.687	2.636	2.552
500	2.295	2.165	2.230	2.146
250	1.594	1.523	1.559	1.475
125	0.889	0.876	0.883	0.799
62.5	0.441	0.454	0.448	0.364
31.2	0.261	0.252	0.257	0.173
15.6	0.143	0.148	0.146	0.062
0	0.083	0.084	0.084	?

Species

Bos taurus

Sensitivity

0.39 ug/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin A,IgA

Applications

ELISA

Research Area

IgA

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Improvements of immune status, intestinal integrity and gain performance in the early-weaned calves parenterally supplemented with l-alanyl-l-glutamine dipeptide /,Veterinary Immunology and Immunopathology,2012 Jan 15;145(1-2):134-42.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price
BHE10500149	CSB-E12018B	96T	$395	Add to Cart
BHE10500149	CSB-E12018B	5x96T	$1896	Add to Cart
BHE10500149	CSB-E12018B	10x96T	$3752.50	Add to Cart

Bovine Immunoglobulin A,IgA ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Bovine Immunoglobulin A,IgA ELISA Kit

Rate it now..!!