Matrices listed below were spiked with certain level of IL1ra and the recovery rates were calculated by comparing the measured value to the expected amount of IL1ra in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
95
EDTA plasma(n=5)
87-105
94
heparin plasma(n=5)
85-101
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL1ra and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-105%
86-102%
89-103%
EDTA plasma(n=5)
91-99%
84-98%
86-100%
heparin plasma(n=5)
80-96%
82-97%
83-98%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
O77482
Antigen
IL1ra
Applications
ELISA
Applications Notes
IL1ra ELISA Kit allows for the in vitro quantitative determination of IL1ra concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Bovine IL1ra(Interleukin 1 Receptor Antagonist) ELISA Kit