Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of IL-15 and the recovery rates were calculated by comparing the measured value to the expected amount of IL-15 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-98
93
EDTA plasma(n=5)
89-98
92
heparin plasma(n=5)
87-105
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IL-15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
89-103%
88-105%
85-104%
EDTA plasma(n=5)
86-101%
88-100%
83-98%
heparin plasma(n=5)
81-95%
80-100%
82-93%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q28028
Applications
ELISA
Applications Notes
IL-15 ELISA Kit allows for the in vitro quantitative determination of IL-15 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Bovine IL-15 (Interleukin-15) ELISA Kit