Matrices listed below were spiked with certain level of IGFBP-5 and the recovery rates were calculated by comparing the measured value to the expected amount of IGFBP-5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-105
94
EDTA plasma(n=5)
87-101
93
heparin plasma(n=5)
86-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IGFBP-5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
87-102%
88-103%
86-100%
EDTA plasma(n=5)
85-100%
89-96%
83-101%
heparin plasma(n=5)
82-93%
82-98%
80-100%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
UniProt ID
Q05717
Applications
ELISA
Applications Notes
IGFBP-5 ELISA Kit allows for the in vitro quantitative determination of IGFBP-5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Bovine IGFBP-5(Insulin Like Growth Factor Binding Protein 5) ELISA Kit