Bovine Heparin-binding growth factor 2(FGF2) ELISA kit

Mfr.No.

CSB-EL008625BO

Sensitivity

1.56 pg/mL

Detection Range

6.25 pg/mL-400 pg/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor 2 (basic), BFGF, FGFB, HBGF-2, basic fibroblast growth factor bFGF, fibroblast growth factor 2, heparin-binding growth factor 2, prostatropin

Recovery

The recovery of rat TNFsR-II spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-96
EDTA plasma (n=4)	98	95-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat TNFsR-II in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	87-94
1:2	Average %	97
	Range %	92-101
1:4	Average %	90
	Range %	87-98
1:8	Average %	92
	Range %	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.827	2.899	2.863	2.732
20	2.222	2.323	2.273	2.142
10	1.373	1.412	1.393	1.262
5	0.816	0.866	0.841	0.710
2.5	0.531	0.545	0.538	0.407
1.25	0.315	0.323	0.319	0.188
0.625	0.226	0.241	0.234	0.103
0	0.126	0.135	0.131	?

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P03969

Applications	ELISA
Research Area	FGF2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500140	CSB-EL008625BO	96T	$595	Add to Cart
BHE10500140	CSB-EL008625BO	5x96T	$2856	Add to Cart
BHE10500140	CSB-EL008625BO	10x96T	$5652.50	Add to Cart

Bovine Heparin-binding growth factor 2(FGF2) ELISA kit

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