Bovine Heat Shock Protein 70,HSP-70 ELISA Kit

Mfr.No.

CSB-E13452B

Alternative Names

Heat Shock Protein 70,HSP-70

Reactivity

Bovine

Detection Range

1.25 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of human Cytochrome P450 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	94-102
EDTA plasma (n=4)	89	84-93

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human Cytochrome P450 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-97
1:2	Average %	104
	Range %	100-112
1:4	Average %	95
	Range %	90-100
1:8	Average %	84
	Range %	80-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.407	2.532	2.470	2.319
1000	1.611	1.684	1.648	1.497
500	1.052	1.005	1.029	0.878
250	0.609	0.593	0.601	0.450
125	0.395	0.419	0.407	0.256
62.5	0.313	0.326	0.320	0.169
31.25	0.268	0.251	0.260	0.109
0	0.154	0.147	0.151	?

Species

Bos taurus

Sensitivity

1.25 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q27975
Antigen	Heat Shock Protein 70,HSP-70

Applications

ELISA

Research Area

HSP70

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effects of transport distance, lairage time and stunning efficiency on cortisol, glucose, HSPA1A and how they relate with meat quality in cattle Chulayo AY.et al,Meat Sci.,2016 117:89-96. Inorganic zinc supplementation modulates heat shock and immune response in heat stressed peripheral blood mononuclear cells of periparturient dairy cows Aasif Ahmad Sheikh.et al,Theriogenology.,42794 Crossbred Cows Respond Differently from Holstein Frisian and Bos Indicus to Heat Stress Under Various Climatic Conditions. Khan, Ikramullah.et al,Sarhad Journal of Agriculture,July

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SKU	Supplier No.	Size	Your price
BHE10500134	CSB-E13452B	96T	$790	Add to Cart
BHE10500134	CSB-E13452B	5x96T	$3792	Add to Cart
BHE10500134	CSB-E13452B	10x96T	$7505	Add to Cart

Bovine Heat Shock Protein 70,HSP-70 ELISA Kit

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