Bovine Haptoglobin,Hpt/HP ELISA KIT

Bovine Haptoglobin,Hpt/HP ELISA KIT

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Mfr.No.

CSB-E08585b

Sensitivity

7.81 ng/mL

Detection Range

7.81 ng/mL-2000 ng/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

haptoglobin, BP, HP2-ALPHA-2, HPA1S, MGC111141, binding peptide, haptoglobin alpha(1S)-beta, haptoglobin alpha(2FS)-beta, haptoglobin, alpha polypeptide, haptoglobin, beta polypeptide

Recovery

The recovery of pig ANG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	98	94-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig ANG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	94
	Range %	90-98
1:200	Average %	96
	Range %	92-99
1:400	Average %	103
	Range %	97-107
1:800	Average %	84
	Range %	80-88

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
6000	0.129	0.128	0.129
3000	0.261	0.256	0.259
1500	0.509	0.529	0.519
750	0.932	0.941	0.937
375	1.401	1.394	1.398
188	1.753	1.742	1.748
94	2.087	2.007	2.047
0	2.476	2.554	2.515

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q2TBU0

Applications	ELISA
Research Area	HP

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500133	CSB-E08585b	10x96T	$8132	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.