Bovine Gonadotropin-releasing Hormone Receptor

Mfr.No.

E0163Bo

Sensitivity

15pg/ml

Reactivity

Bovine

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Gonadotropin-releasing hormone receptor (also known as GnRHR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

GnRH receptor;GNRHR;GnRH-R;Gonadotropin-releasing hormone receptor

Molecular Weight

37741

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0163Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1243.8	76.05	6.1
Intra-Assay	2	18	2269.2	61.78	2.7
Intra-Assay	3	18	964.8	63.53	6.6

Background

Receptor for gonadotropin releasing hormone (GnRH) that mediates the action of GnRH to stimulate the secretion of the gonadotropic hormones luteinizing hormone (LH) and follicle-stimulating hormone (FSH). This receptor mediates its action by association with G-proteins that activate a phosphatidylinositol-calcium second messenger system.

Protocol

These standard curves of E0163Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800pg/ml	2.344	2.305	2.251
	2.267
2400pg/ml	1.256	1.3	1.246
	1.344
1200pg/ml	0.827	0.867	0.813
	0.907
600pg/ml	0.525	0.467	0.412
	0.409
300pg/ml	0.234	0.275	0.22
	0.315
0pg/ml	0.047	0.054	0
	0.062

Gene ID	281798
UniProt ID	P32236
Antigen	GNRHR

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100174	E0163Bo	96T	$458		Add to Cart
BHE12100174	E0163Bo	48T	$320		Add to Cart

Bovine Gonadotropin-releasing Hormone Receptor - GNRHR ELISA Kit

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