Bovine gonadotropin-releasing hormone(GnRH)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E15857B

Sensitivity

100 pg/mL

Detection Range

100 pg/mL-4000 pg/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

gonadotropin-releasing hormone(GnRH)

Recovery

The recovery of mouse MBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-100
EDTA plasma (n=4)	99	94-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse MBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	82-97
1:2	Average %	96
	Range %	91-104
1:4	Average %	97
	Range %	95-106
1:8	Average %	95
	Range %	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q0VBW7

Applications	ELISA
Research Area	GnRH

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Biosynthesis of gonadotropin-releasing hormone (GnRH) and GnRH receptor (GnRHR) in hypothalamic-pituitary unit of anoestrous and cyclic ewes M.O. Ciechanowska.et al,Canadian Journal of Physiology and Pharmacology,Nov 30

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