Bovine Gamma-Interferon-inducible Lysosomal Thiol Reductase

Mfr.No.

E0329Bo

Sensitivity

0.01ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Bovine Gamma-interferon-inducible lysosomal Thiol Reductase (also known as IFI30) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Gamma-interferon-inducible lysosomal thiol reductase;IFI 30;IFI30;IFI30 protein

Molecular Weight

27490

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0329Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.2	0.14	4.4
Intra-Assay	2	18	5.5	0.12	2.2
Intra-Assay	3	18	2.9	0.13	4.7

Background

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation (By similarity).

Protocol

These standard curves of E0329Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.141	2.069	2.018
	1.997
4ng/ml	0.932	0.939	0.887
	0.945
2ng/ml	0.491	0.482	0.431
	0.473
1ng/ml	0.319	0.247	0.195
	0.175
0.5ng/ml	0.164	0.147	0.096
	0.13
0ng/ml	0.054	0.051	0
	0.048

Gene ID	615930
UniProt ID	A6QPN6

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100306	E0329Bo	96T	$458		Add to Cart
BHE12100306	E0329Bo	48T	$320		Add to Cart

Bovine Gamma-Interferon-inducible Lysosomal Thiol Reductase - IFI30 ELISA Kit

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