Bovine Fibulin 5 - FBLN5 ELISA Kit

Mfr.No.

E2080Bo

Sensitivity

0.019ng/ml

Detection Range

0.05-15ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Bovine Fibulin 5 (also known as FBLN5) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FBLN 5;FBLN5;FIBL-5;Fibulin-5

Molecular Weight

50164

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2080Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2.3	0.14	6
Intra-Assay	2	18	5	0.16	3.2
Intra-Assay	3	18	1.7	0.1	6

Background

Essential for elastic fiber formation, is involved in the assembly of continuous elastin (ELN) polymer and promotes the interaction of microfibrils and ELN. Stabilizes and organizes elastic fibers in the skin, lung and vasculature. Promotes adhesion of endothelial cells through interaction of integrins and the RGD motif. Vascular ligand for integrin receptors which may play a role in vascular development and remodeling. May act as an adapter that mediates the interaction between FBN1 and ELN.

Protocol

These standard curves of E2080Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/ml	2.233	2.298	2.267
	2.362
4ng/ml	1.175	1.27	1.239
	1.365
2ng/ml	0.837	0.833	0.803
	0.83
1ng/ml	0.52	0.465	0.434
	0.41
0.5ng/ml	0.247	0.246	0.215
	0.244
0ng/ml	0.034	0.031	0
	0.027

Gene ID	535185
UniProt ID	Q5EA62

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100450	E2080Bo	96T	$458		Add to Cart
BHE12100450	E2080Bo	48T	$320		Add to Cart

Bovine Fibulin 5 - FBLN5 ELISA Kit

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