Bovine Fibronectin,FN ELISA Kit

Bovine Fibronectin,FN ELISA Kit

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Mfr.No.

CSB-E13781B

Alternative Names

fibronectin 1, CIG, DKFZp686F10164, DKFZp686H0342, DKFZp686I1370, DKFZp686O13149, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, OTTHUMP00000206762, OTTHUMP00000206767, cold-insoluble globulin, migration-stimulating fa

Reactivity

Bovine

Detection Range

0.137 ug/mL-100 ug/mL

Sample Type

Cell lysates, Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TNN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	92	86-95

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TNN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-99
1:2	Average %	102
	Range %	97-105
1:4	Average %	94
	Range %	88-98
1:8	Average %	98
	Range %	93-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.022	2.001	2.012	1.855
5	1.733	1.812	1.773	1.616
2.5	1.307	1.288	1.298	1.141
1.25	0.989	0.973	0.981	0.824
0.625	0.673	0.666	0.670	0.513
0.312	0.466	0.454	0.460	0.303
0.156	0.301	0.312	0.307	0.150
0	0.156	0.158	0.157

Species

Bos taurus

Sensitivity

0.137 ug/mL

Limit Of Detection

450 nm

UniProt ID	P07589
Antigen	Fibronectin 1

Applications

ELISA

Research Area

FN1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500115	CSB-E13781B	96T	$595	Add to Cart
BHE10500115	CSB-E13781B	5x96T	$2856	Add to Cart
BHE10500115	CSB-E13781B	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.