Bovine Fibrinogen,Fb Elisa Kit

Mfr.No.

CSB-E13283B

Sensitivity

1.56 ug/mL

Detection Range

3.125 ug/mL-200 ug/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

Fibrinogen,Fb

Recovery

The recovery of rat ATP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	98	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat ATP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	83-90
1:2	Average %	102
	Range %	98-106
1:4	Average %	86
	Range %	83-89
1:8	Average %	98
	Range %	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

Applications	ELISA
Research Area	Fb

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Acute Phase Response in Enzootic Bovine Leukosis Guzel, M.et al,Journal of the Hellenic Veterinary Medical Society,/

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