Bovine Fetuin B - FETU-B ELISA Kit

Mfr.No.

E2017Bo

Sensitivity

2.48ng/ml

Detection Range

5-2000ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Fetuin B (also known as FETU-B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

FETUB;Fetuin-B;Gugu

Molecular Weight

42663

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2017Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	162.9	10.93	6.7
Intra-Assay	2	18	516.3	22.82	4.4
Intra-Assay	3	18	346.7	25.51	7.4

Background

Protease inhibitor required for egg fertilization. Required to prevent premature zona pellucida hardening before fertilization, probably by inhibiting the protease activity of ASTL, a protease that mediates the cleavage of ZP2 and triggers zona pellucida hardening (By similarity).

Protocol

These standard curves of E2017Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/ml	2.16	2.166	2.111
	2.173
600ng/ml	1.674	1.615	1.56
	1.556
300ng/ml	1.195	1.12	1.065
	1.044
150ng/ml	0.647	0.647	0.592
	0.648
75ng/ml	0.456	0.426	0.372
	0.397
0ng/ml	0.056	0.055	0
	0.054

Gene ID	504615
UniProt ID	Q58D62

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100398	E2017Bo	96T	$458		Add to Cart
BHE12100398	E2017Bo	48T	$320		Add to Cart

Bovine Fetuin B - FETU-B ELISA Kit

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