Bovine Estrogen Receptor Beta - ER-beta ELISA Kit

Mfr.No.

E2019Bo

Sensitivity

0.024ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Bovine Estrogen Receptor beta (also known as ER-beta) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ER-beta;ESR 2;ESR2;Estrogen receptor beta;Nuclear receptor subfamily 3 group A member 2

Molecular Weight

59032

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2019Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.2	0.21	6.6
Intra-Assay	2	18	8.6	0.38	4.4
Intra-Assay	3	18	2.3	0.14	6

Background

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1 (ER-alpha), and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. May play a role in ovarian follicular growth and maturation.

Protocol

These standard curves of E2019Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.144	2.194	2.142
	2.244
6ng/ml	1.433	1.413	1.361
	1.394
3ng/ml	0.8	0.765	0.713
	0.73
1.5ng/ml	0.471	0.499	0.447
	0.527
0.75ng/ml	0.248	0.249	0.197
	0.25
0ng/ml	0.07	0.052	0
	0.034

Gene ID	281146
UniProt ID	Q9XSB5

Research Area	Epigenetics and Nuclear Signaling

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100400	E2019Bo	96T	$458		Add to Cart
BHE12100400	E2019Bo	48T	$320		Add to Cart

Bovine Estrogen Receptor Beta - ER-beta ELISA Kit

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