Bovine Endothelin 1 - ET-1 ELISA Kit

Mfr.No.

E0122Bo

Sensitivity

1.19ng/L

Detection Range

2-600ng/L

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Big endothelin-1;EDN 1;EDN1;Endothelin-1;ET-1;PPET1;Preproendothelin-1

Molecular Weight

22977

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0122Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	101.4	6.29	6.2
Intra-Assay	2	18	153.7	6.45	4.2
Intra-Assay	3	18	50.3	2.44	4.8

Background

Endothelins are endothelium-derived vasoconstrictor Peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (By similarity).

Protocol

These standard curves of E0122Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
320ng/L	2.175	2.136	2.08
	2.097
160ng/L	1.398	1.404	1.348
	1.41
80ng/L	0.712	0.74	0.684
	0.767
40ng/L	0.459	0.447	0.392
	0.436
20ng/L	0.269	0.252	0.196
	0.235
0ng/L	0.063	0.056	0
	0.049

Gene ID	281137
UniProt ID	P17322

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100133	E0122Bo	96T	$458		Add to Cart
BHE12100133	E0122Bo	48T	$320		Add to Cart

Bovine Endothelin 1 - ET-1 ELISA Kit

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