Bovine Ectonucleoside Triphosphate Diphosphohydrolase 1

Mfr.No.

E2206Bo

Sensitivity

9.72ng/L

Detection Range

20-6000ng/L

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

CD antigen CD39;Ecto-apyrase;Ecto-ATP diphosphohydrolase 1;Ecto-ATPase 1;Ecto-ATPDase 1;Ectonucleoside triphosphate diphosphohydrolase 1;ENTPD1;Lymphoid cell activation antigen;NTPDase 1;

Molecular Weight

58114

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E2206Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	473.15	28.93	6.11
Intra-Assay	2	18	1735	84.71	4.88
Intra-Assay	3	18	248.24	23.6	9.51

Background

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP. Hydrolyzes ATP and ADP equally well.

Protocol

These standard curves of E2206Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.272	2.193	2.142
	2.114
1600ng/L	1.53	1.608	1.557
	1.686
800ng/L	1.051	1.032	0.982
	1.014
400ng/L	0.72	0.68	0.629
	0.639
200ng/L	0.492	0.479	0.428
	0.466
0ng/L	0.05	0.051	0
	0.051

Gene ID	282223
UniProt ID	O18956

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100500	E2206Bo	48T	$320		Add to Cart
BHE12100500	E2206Bo	96T	$458		Add to Cart

Bovine Ectonucleoside Triphosphate Diphosphohydrolase 1 - ENTPD1 ELISA Kit

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