Bovine Claudin-3 - CLDN3 ELISA Kit

Mfr.No.

E0370Bo

Sensitivity

3.62ng/L

Detection Range

7-2500ng/L

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Claudin-3 (also known as CLDN3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Claudin-3;CLDN 3;CLDN3

Molecular Weight

23382

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0370Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	358.4	15.29	4.3
Intra-Assay	2	18	526.6	16.46	3.1
Intra-Assay	3	18	140	9	6.4

Background

Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium-independent cell-adhesion activity.

Protocol

These standard curves of E0370Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1280ng/L	2.527	2.517	2.46
	2.506
640ng/L	1.503	1.465	1.408
	1.427
320ng/L	0.899	0.889	0.832
	0.878
160ng/L	0.459	0.467	0.411
	0.475
80ng/L	0.206	0.245	0.189
	0.285
0ng/L	0.061	0.057	0
	0.052

Gene ID	404153
UniProt ID	Q765N9

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100338	E0370Bo	96T	$458		Add to Cart
BHE12100338	E0370Bo	48T	$320		Add to Cart

Bovine Claudin-3 - CLDN3 ELISA Kit

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