Bovine Beta-Endorphin,beta-EP ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13051B

Alternative Names

Beta-Endorphin,beta-EP, beta-Endorphin, beta-EP

Reactivity

Bovine

Detection Range

46.88 pg/mL-3000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human SFRP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	85	82-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human SFRP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	83
	Range %	80-86
1:200	Average %	95
	Range %	90-101
1:400	Average %	96
	Range %	93-100
1:800	Average %	90
	Range %	85-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Bos taurus

Sensitivity

11.72 pg/mL

Limit Of Detection

450 nm

Antigen	Beta-Endorphin,β-EP

Applications

ELISA

Research Area

beta-EP

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10500043	CSB-E13051B	96T	$595	Add to Cart
BHE10500043	CSB-E13051B	5x96T	$2856	Add to Cart
BHE10500043	CSB-E13051B	10x96T	$5652.50	Add to Cart