Bovine Beta-defensin 1 - DEFB1 ELISA Kit

Mfr.No.

E0111Bo

Sensitivity

0.54ng/ml

Detection Range

1-400ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Beta-defensin 1;BNBD-1;BNDB-1;DEFB1

Molecular Weight

4278

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0111Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	159.53	3.68	2.31
Intra-Assay	2	18	62.93	3.24	5.16
Intra-Assay	3	18	44.52	2.88	6.47

Background

Has bactericidal activity. Active against E.coli ML35 but not against S.aureus 502A (PubMed:8454635). May act as a ligand for C-C chemokine receptor CCR6. Positively regulates the sperm motility and bactericidal activity in a CCR6-dependent manner. Binds to CCR6 and triggers Ca2+ mobilization in the sperm which is important for its motility (By similarity).

Protocol

These standard curves of E0111Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
240ng/ml	2.366	2.385	2.333
	2.404
120ng/ml	1.485	1.435	1.382
	1.385
60ng/ml	0.895	0.881	0.829
	0.867
30ng/ml	0.392	0.46	0.407
	0.528
15ng/ml	0.25	0.247	0.195
	0.244
0ng/ml	0.051	0.053	0
	0.054

Gene ID	100297617
UniProt ID	P46159

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100122	E0111Bo	48T	$320		Add to Cart
BHE12100122	E0111Bo	96T	$458		Add to Cart

Bovine Beta-defensin 1 - DEFB1 ELISA Kit

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