Bovine Apolipoprotein H - APO-H ELISA Kit

Mfr.No.

E0086Bo

Sensitivity

0.11ug/ml

Reactivity

Bovine

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Apolipoprotein H/Beta-2-glycoprotein 1 (also known as Apo-H) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

APOH;Apo-H;Apolipoprotein H;B2GPI;Beta,2GPI;Beta-2-glycoprotein 1;Beta-2-glycoprotein I

Molecular Weight

38252

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0086Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.9	0.67	3.8
Intra-Assay	2	18	13.4	0.76	5.6
Intra-Assay	3	18	6	0.33	5.4

Background

Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Protocol

These standard curves of E0086Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ug/ml	2.219	2.114	2.072
	2.009
20ug/ml	1.493	1.464	1.422
	1.435
10ug/ml	0.758	0.72	0.678
	0.683
5ug/ml	0.357	0.361	0.319
	0.365
2.5ug/ml	0.176	0.195	0.153
	0.215
0ug/ml	0.047	0.042	0
	0.037

Gene ID	281006
UniProt ID	P17690
Antigen	APOH

Research Area	Cardiovascular; Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100098	E0086Bo	96T	$458		Add to Cart
BHE12100098	E0086Bo	48T	$320		Add to Cart

Bovine Apolipoprotein H - APO-H ELISA Kit

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