Bovine Apolipoprotein A-I(APOA1) ELISA kit

Mfr.No.

CSB-EL001913BO

Sensitivity

19.5 ng/mL

Detection Range

78 ng/mL-5000 ng/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Alternative Names

apolipoprotein A-I, MGC117399, OTTHUMP00000069346, OTTHUMP00000069347, OTTHUMP00000069348

Recovery

The recovery of pig trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-103
EDTA plasma (n=4)	99	94-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:5

Average %

Range %

83-97

1:10

Average %

Range %

80-90

1:20

Average %

Range %

84-99

1:40

Average %

Range %

88-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.192	2.131	2.162	2.029
100	1.769	1.834	1.802	1.669
50	1.289	1.319	1.304	1.171
25	0.824	0.867	0.846	0.713
12.5	0.543	0.577	0.560	0.427
6.25	0.372	0.337	0.355	0.222
3.12	0.288	0.259	0.274	0.141
0	0.137	0.129	0.133	?

Species

Bos taurus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P15497

Applications	ELISA
Research Area	APOA1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10500030	CSB-EL001913BO	96T	$595	Add to Cart
BHE10500030	CSB-EL001913BO	5x96T	$2856	Add to Cart
BHE10500030	CSB-EL001913BO	10x96T	$5652.50	Add to Cart

Bovine Apolipoprotein A-I(APOA1) ELISA kit

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