Bovine Anti-Thrombin 3 - AT-3 ELISA Kit

Mfr.No.

E0195Bo

Sensitivity

10.04ng/ml

Detection Range

20-6000ng/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Bovine Anti-Thrombin Ⅲ (also known as AT-III) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Antithrombin-III;AT3;ATIII;Serpin C1;SERPINC 1;SERPINC1;THPH7

Molecular Weight

52347

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0195Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2630.8	48.6	1.8
Intra-Assay	2	18	752.4	34.78	4.6
Intra-Assay	3	18	1221.1	41.38	3.4

Background

Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. AT-III inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin (By similarity).

Protocol

These standard curves of E0195Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/ml	1.936	2.045	2.005
	2.154
1600ng/ml	1.014	1.037	0.997
	1.06
800ng/ml	0.387	0.468	0.429
	0.549
400ng/ml	0.287	0.243	0.203
	0.199
200ng/ml	0.141	0.106	0.067
	0.072
0ng/ml	0.037	0.04	0
	0.043

Gene ID	540261
UniProt ID	P41361

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100205	E0195Bo	96T	$458		Add to Cart
BHE12100205	E0195Bo	48T	$320		Add to Cart

Bovine Anti-Thrombin 3 - AT-3 ELISA Kit

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