Bovine adiponectin,ADP ELISA Kit

Bovine adiponectin,ADP ELISA Kit

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Mfr.No.

CSB-E14054B

Sensitivity

3.125 ug/mL

Detection Range

3.125 ug/mL-50 ug/mL

Reactivity

Bovine

Assay Duration

1-5h

Assay Type

Competitive

Sample Volume

50-100ul

Alternative Names

adiponectin, C1Q and collagen domain containing, ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin, OTTHUMP00000210047, OTTHUMP00000210048, adipocyte, C1Q and collagen domain containing, adipocyte, C1Q and collagen domain-containing, adipo

Recovery

The recovery of human FOLH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	89-105
EDTA plasma (n=4)	96	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human FOLH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	92
	Range %	85-98
1:10	Average %	95
	Range %	85-100
1:20	Average %	96
	Range %	89-103
1:40	Average %	89
	Range %	81-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
300	2.871	2.889	2.880	2.776
150	2.529	2.532	2.531	2.427
75	1.882	1.897	1.890	1.786
37.5	1.174	1.165	1.170	1.066
18.8	0.699	0.706	0.703	0.599
9.4	0.482	0.501	0.492	0.388
4.7	0.308	0.315	0.312	0.208
0	0.106	0.102	0.104	?

Species

Bos taurus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q3Y5Z3

Applications	ELISA
Research Area	ADIPOQ

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of endoplasmic reticulum stress on metabolic and stress signaling and kidney-specific functions in Madin-Darby bovine kidney cells Chiappisi E.et al,J Dairy Sci.,Jun 14

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.