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Home / ELISA Kit

Bovine Acetylcholinesterase - ACHE ELISA Kit

SKU : BHE12100193

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

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Bioassay Technology Laboratory

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+1 866.986.9598

Email

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Mfr.No.

E0183Bo

Sensitivity

0.28U/ml

Detection Range

0.5-100U/ml

Reactivity

Bovine

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Acetylcholinesterase;AChE

Molecular Weight

67664

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0183Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	42.3	1.84	4.3
Intra-Assay	2	18	18.4	0.96	5.2
Intra-Assay	3	18	6.3	0.36	5.8

Background

Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.

Protocol

These standard curves of E0183Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
64U/ml	2.162	2.186	2.131
	2.21
32U/ml	1.37	1.455	1.4
	1.54
16U/ml	0.884	0.806	0.751
	0.728
8U/ml	0.459	0.488	0.433
	0.516
4U/ml	0.227	0.248	0.193
	0.269
0U/ml	0.067	0.055	0
	0.043

Gene ID	540446
UniProt ID	P23795

Research Area	Neuroscience; Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12100193	E0183Bo	96T	$458		Add to Cart
BHE12100193	E0183Bo	48T	$320		Add to Cart

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