Matrices listed below were spiked with certain level of ANG I and the recovery rates were calculated by comparing the measured value to the expected amount of ANG I in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
97
EDTA plasma(n=5)
85-95
90
heparin plasma(n=5)
85-103
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ANG I and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-95%
89-101%
93-102%
EDTA plasma(n=5)
84-101%
82-98%
89-98%
heparin plasma(n=5)
84-97%
84-100%
82-100%
Sensitivity
< 0.188ng/ml
Notes
For Research Use Only
Antigen
ANG I
Applications
ELISA
Applications Notes
ANG I ELISA Kit allows for the in vitro quantitative determination of ANG I concentrations in serum, plasma, tissue homogenates and other biological fluids.