Matrices listed below were spiked with certain level of ALD and the recovery rates were calculated by comparing the measured value to the expected amount of ALD in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-103
93
EDTA plasma(n=5)
91-101
96
heparin plasma(n=5)
85-103
91
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ALD and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
ALD ELISA Kit allows for the in vitro quantitative determination of ALD concentrations in serum, plasma, tissue homogenates and other biological fluids.