Matrices listed below were spiked with certain level of 3-NT and the recovery rates were calculated by comparing the measured value to the expected amount of 3-NT in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-103
99
EDTA plasma(n=5)
85-105
97
heparin plasma(n=5)
89-101
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 3-NT and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
3-NT ELISA Kit allows for the in vitro quantitative determination of 3-NT concentrations in serum, plasma, tissue homogenates and other biological fluids.