25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit

Mfr.No.

EU2544

Description

25-HVD3(25-Hydroxy Vitamin D3) ELISA Kit

Alternative Names

25-HVD3 ELISA Kit, 25-Hydroxy Vitamin D3 ELISA Kit

Reactivity

Universal

Detection Method

Coated with Antigen

Detection Range

0.781-50ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Competitive

Recovery

Matrices listed below were spiked with certain level of 25-HVD3 and the recovery rates were calculated by comparing the measured value to the expected amount of 25-HVD3 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-105	97
EDTA plasma(n=5)	89-104	98
heparin plasma(n=5)	88-104	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-HVD3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-105%	90-99%	87-104%
EDTA plasma(n=5)	84-100%	85-92%	83-94%
heparin plasma(n=5)	82-99%	86-92%	85-100%

Sensitivity

< 0.469ng/ml

Notes

For Research Use Only

Applications	ELISA
Applications Notes	25-HVD3 ELISA Kit allows for the in vitro quantitative determination of 25-HVD3 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10808028	EU2544	96T	$450		Add to Cart

25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit

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