25-HVD2(25-Hydroxy Vitamin D2) ELISA Kit

Mfr.No.

EU2546

Description

25-HVD2(25-Hydroxy Vitamin D2) ELISA Kit

Alternative Names

25-HVD2 ELISA Kit, 25-Hydroxy Vitamin D2 ELISA Kit

Reactivity

Universal

Detection Method

Coated with Antigen

Detection Range

0.781-50ng/ml

Sample Type

Cell lysate, Tissue lysate, Other liquid samples, Serum, Plasma, Cell culture supernatants

Assay Type

Competitive

Recovery

Matrices listed below were spiked with certain level of 25-HVD2 and the recovery rates were calculated by comparing the measured value to the expected amount of 25-HVD2 in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	90-104	99
EDTA plasma(n=5)	91-99	96
heparin plasma(n=5)	89-101	93

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-HVD2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	87-100%	85-100%	85-100%
EDTA plasma(n=5)	86-99%	88-98%	87-98%
heparin plasma(n=5)	95-99%	81-100%	87-100%

Sensitivity

< 0.469ng/ml

Notes

For Research Use Only

Applications	ELISA
Applications Notes	25-HVD2 ELISA Kit allows for the in vitro quantitative determination of 25-HVD2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10808029	EU2546	96T	$450		Add to Cart

25-HVD2(25-Hydroxy Vitamin D2) ELISA Kit

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