Matrices listed below were spiked with certain level of 16,16-Dimethyl-PGE2 and the recovery rates were calculated by comparing the measured value to the expected amount of 16,16-Dimethyl-PGE2 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-100
95
EDTA plasma(n=5)
89-105
94
heparin plasma(n=5)
90-102
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 16,16-Dimethyl-PGE2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
93-104%
88-96%
90-103%
EDTA plasma(n=5)
95-99%
83-101%
82-100%
heparin plasma(n=5)
80-97%
88-100%
81-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
16,16-Dimethyl-PGE2 ELISA Kit allows for the in vitro quantitative determination of 16,16-Dimethyl-PGE2 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of 16,16-Dimethyl-PGE2(16,16-dimethyl Prostaglandin E2) ELISA Kit