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Home / ELISA Kit

EliKine Rat TNF-alpha ELISA Kit

SKU : BHE13710200

Tumor necrosis factor acts as a cytokine, binding TNF receptors and plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE9007
Description	EliKine Rat TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Rat TNF-alpha.
Alternative Names	TNF-alpha; TNF; DADB-70P7.1; DIF; TNF-alpha; TNFA; TNFSF2; APC1 protein; TNF superfamily; member 2; TNF; macrophage-derived; TNF; monocyte-derived; cachectin; tumor necrosis factor alpha; TNFSF7
Reactivity	Rat
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Rat TNF-alpha microplate Rat TNF-alpha standard Rat TNF-alpha detect antibody EliKine Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	EliKine Rat TNF-alpha ELISA Kit has high sensitivity and excellent specificity for detection of Rat TNF-alpha. No significant cross-reactivity or interference between Rat TNF-alpha and analogues was observed.
Calibration Range	31.25 pg/mL- 2000 pg/mL
Limit Of Detection	16 pg/ml
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tumor necrosis factor acts as a cytokine, binding TNF receptors and plays a role in regulation of cell proliferation, induction of apoptosis, and inflammatory response.
Notes	EliKine Rat TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-alpha in samples. An antibody specific for TNF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-alpha is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	24835
Tag	TNFA
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	EliKine Rat TNF-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate TNF-alpha in samples. An antibody specific for TNF-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNF-alpha is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNF-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710200	KTE9007	48 T	$189		Add to Cart
BHE13710200	KTE9007	96 T	$289		Add to Cart

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