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Home / ELISA Kit

EliKine Human IFN-alpha ELISA Kit

SKU : BHE13710151

The protein encoded by IFN-alpha gene is produced by macrophages and has antiviral activity. IFN-alpha gene is intronless and the encoded protein is secreted.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE6010
Description	EliKine Human IFN-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate Human IFN-alpha.
Alternative Names	IFNA1; IFL; IFN; IFNA; IFNA13; IFN-ALPHA; IFN-alphaD
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human IFN-alpha microplate Human IFN-alpha standard Human IFN-alpha detect antibody EliKine Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	EliKine Human IFN-alpha ELISA Kit has high sensitivity and excellent specificity for detection of Human IFN-alpha. No significant cross-reactivity or interference between Human IFN-alpha and analogues was observed.
Calibration Range	46.9 pg/mL - 3000 pg/mL
Limit Of Detection	46.9 pg/mL
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	The protein encoded by IFN-alpha gene is produced by macrophages and has antiviral activity. IFN-alpha gene is intronless and the encoded protein is secreted.
Notes	EliKine Human IFN-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-alpha in samples. An antibody specific for IFN-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-alpha is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	3439
Tag	IFN
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	EliKine Human IFN-alpha ELISA Kit employs a two-site sandwich ELISA to quantitate IFN-alpha in samples. An antibody specific for IFN-alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-alpha is added to the wells. After washing, proprietary EliKine Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13710151	KTE6010	48 T	$189		Add to Cart
BHE13710151	KTE6010	96 T	$289		Add to Cart

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