EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

Mfr.No.

ELK052ES

Alternative Names

25 Hydroxy Vitamin D;

Reactivity

Mouse

Detection Range

78.13-5000 ng/mL

Sample Type

Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

1.5h

Recovery

Matrices listed below were spiked with certain level of recombinant 25-OH-D and the recovery rates were calculated by comparing the measured value to the expected amount of 25-OH-D in samples.

Matrix	Recovery range	Average
serum(n=5)	95-107%	101%
EDTA plasma(n=5)	82-95%	88%
Heparin plasma(n=5)	78-96%	87%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of 25-OH-D and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	93-102%	81-96%	93-106%	82-95%
EDTA plasma(n=5)	86-97%	83-96%	91-103%	96-105%
Heparin plasma(n=5)	85-100%	80-97%	81-92%	87-103%

Sensitivity

7.7 ng/mL

Limit Of Detection

5000 ng/mL

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse 25-OH-D antigen, and the Mouse 25-OH-D standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse 25-OH-D to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Mouse 25-OH-D in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area	Reproductive science;Genetic science;Nutrition metabolism;Bone metabolism;
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE15208852	ELK052ES	48T	$320		Add to Cart
BHE15208852	ELK052ES	96T	$370		Add to Cart

EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of EasyStep Mouse 25-OH-D(25 Hydroxy Vitamin D) ELISA Kit

Rate it now..!!