EasyStep Human GDF15(Growth Differentiation Factor 15) ELISA Kit

Mfr.No.

ELK044ES

Alternative Names

PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor

Reactivity

Human

Detection Range

46.88-3000 pg/mL

Sample Type

Serum, Plasma

Assay Type

Sandwich

Assay Duration

1.5h

Recovery

Matrices listed below were spiked with certain level of recombinant GDF15 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF15 in samples.

Matrix	Recovery range	Average
serum(n=5)	87-99%	94%
EDTA plasma(n=5)	84-95%	88%
Heparin plasma(n=5)	82-97%	92%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of GDF15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-102%	82-96%	87-98%	78-92%
EDTA plasma(n=5)	87-103%	97-105%	79-90%	87-96%
Heparin plasma(n=5)	85-95%	85-96%	93-99%	87-101%

Sensitivity

5.22 pg/mL

Limit Of Detection

3000 pg/mL

Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF15, and the Human GDF15 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human GDF15. After TMB substrate solution is added, only those wells that contain Human GDF15 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF15 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID	Q99988

Research Area	Cytokine;Apoptosis;Tumor immunity;Infection immunity;
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208844	ELK044ES	48T	$320		Add to Cart
BHE15208844	ELK044ES	96T	$370		Add to Cart

EasyStep Human GDF15(Growth Differentiation Factor 15) ELISA Kit

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