EasyStep Human fT3(Free Triiodothyronine) ELISA Kit

Mfr.No.

ELK048ES

Alternative Names

3,3',5-Triiodo-L-Thyronine; Liothyronine; Cytomel; Tertroxin

Reactivity

Human

Detection Range

0.32-20 ng/mL

Sample Type

Serum, Plasma

Assay Type

Competitive Inhibition

Assay Duration

1.5h

Recovery

Matrices listed below were spiked with certain level of recombinant fT3 and the recovery rates were calculated by comparing the measured value to the expected amount of fT3 in samples.

Matrix	Recovery range	Average
serum(n=5)	81-95%	88%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	85-97%	91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of fT3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-97%	93-106%	83-95%	88-103%
EDTA plasma(n=5)	95-103%	88-96%	81-95%	91-102%
Heparin plasma(n=5)	86-93%	91-105%	87-99%	99-105%

Sensitivity

0.31 ng/mL

Limit Of Detection

20 ng/mL

Notes

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human fT3 antigen, and the Human fT3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human fT3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Human fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Area	Endocrinology;Autoimmunity;
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15208848	ELK048ES	48T	$320		Add to Cart
BHE15208848	ELK048ES	96T	$370		Add to Cart

EasyStep Human fT3(Free Triiodothyronine) ELISA Kit

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