Human DYRK2 (GenBank Accession No. NM_003583), full length with N-terminal His-tag, MW = 63.5 kDa, expressed in Baculovirus infected Sf9 cell expression system.
host species: Sf9 cells
Host species: Sf9 cells
Specific activity: 111 pmoles/min/ug
Immunogen Region
full length
Tag
N-terminal His-tag
Formulation
25 mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme reaction is conducted in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, and 2 uM substrate (SER/THR 9, Invitrogen) at room temperature for 1 hour.