Alcian Blue solution,pH 2.5

Alcian Blue is a dye that stains sulfated proteoglycanpresent in cartilage tissues, which is an indicator cell chondrogenesis.At pH 1.0 it stains only sulfated polysaccharides; at pH 2.5also stains carboxyl group containing sugars such as sialic acids and uronicacids intensify the stain of hyaluronic acids, which would also stain albeitrelatively weakly by their half sulfate esters at pH 1.0.

At pH 2.5,Alcian Blue stains acid mucopolysaccharides and shows a blue color, whileNuclear Fast Red stains nuclei pink to red and cytoplasm pale pink.

Results

Acid mucins/ mucosubstances( Sulfated Mucosubstances and Sialomucins)      Blue

Proteoglycan and HyaluronicAcid                                                                                 Blue

Nuclei                                                                                                                                  Red