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Home / Assay Kits / Drug Target Assays

HIV Reverse Transcriptase Assay Kit

SKU : BHE16300037

This product includes 400 ul of 10 x Buffer, 33 ul of 100 x Template, 33 ul of 100 x dNTPs, 1550 ul of 2 x Dye and 1550 ul of 50 mM EDTA. It is for 100 assays of HIV Reverse Transcriptase. The assay This product includes all reagents except the enzyme.

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Mfr.No.	HIV100K , HIV100KE
Description	Infection of human immunodeficiency virus (HIV) causes acquired immunodeficiency syndrome (AIDS). The HIV reverse transcriptase (HIV RT) is an attractive drug target for HIV drug discovery. The HIV RT enzyme synthesizes a complementary DNA strand initiating from a primer using either RNA or single-stranded DNA as a template. The HIV Reverse Transcriptase Assay is based on measurement of the DNA molecules synthesized by HIV RT. The assay can be performed in a 384-well or 96-well plate format for tests of HIV reverse transcriptase activities and high throughput screening of inhibitors. The HIV Reverse Transcriptase Assay Kit (Catalog number: HIV100K) includes 400 µl of 10 x Buffer, 33 µl of 100 x Template, 33 µl of 100 x dNTPs, 1550 µl of 2 x Dye and 1550 µl of 50 mM EDTA. It is for 100 assays of HIV Reverse Transcriptase. The assay kit includes all reagents except the enzyme.

Product Manual	Datasheet
Protocols Description	ASSAY PROTOCOL The following assay protocol is based on the 384-well plate assay format (plate type: Matrix 4318 or alike). The reaction volume is 30 µl and the final assay volume is 60 µl. For 96-well plate assays (plate type: Costar 3915 or alike), the reaction volume is 60 µl and the final assay volume is 120 µl. 1. Reagent preparation: (1) 10 x Template: Dilute the 100 x Template 10-fold with water. Each assay uses 3 µl of 10 x Template. (2) 10 x enzyme: Dilute the 500 x HIV RT (2 Unit / µl) 50-fold with the 1 x assay buffer. Each assay uses 3 µl of 10 x enzyme. (3) 10 x dNTP mix: Dilute the 100 x dNTP mix (1 mM dATP, 1 mM dGTP) 10-fold with water. Each assay uses 3 µl of 10 x dNTP mix. (4) 1 x dye: Dilute the 2 x fluorescence dye 2-fold with 50 mM EDTA. Each assay uses 30 µl of 1 x dye. 2. Reaction: The total volume of each reaction mixture is 30 µl including 18 µl of H2O, 3 µl of 10 x Buffer, 3 µl of 10 x template, 3 µl of 10 x enzyme, 3 µl of 10 x dNTP mix. Incubate the reaction mixture at 37°C for 60 min. 3. Detection: Mix 30 µl of the 1 x fluorescence dye with 30 µl of the reaction mixture. Measure the fluorescence intensity at 535 nm using the excitation wavelength at 485 nm.

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Combination SKU	Supplier No.	Specifications	Size	Your price	Quantity
BHE16300037-1	HIV100K	enzyme not included	100 assays	$378.19		Add to Cart
BHE16300037-2	HIV100KE	enzyme included	100 assays	$634.60		Add to Cart

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