mRNA Vaccinia Capping Enzyme (10 U/uL)

Mfr.No.

10615ES92, 10615ES84

Description

Eukaryotes mRNA forms a special structure at the 5'end after transcription, which is the cap structure. The cap structure plays an important role in the stability, transportation, and translation of mRNA. The vaccinia virus capping enzyme is an effective enzyme that can catalyze the formation of the cap structure. It"s composed of two subunits D1 and D12. It also has RNA triphosphatase activity, guanylate acyltransferase activity, and guanine methyltransferase activity that could connect the 7-methylguanine cap structure (m7Gppp) to the 5'end of the RNA (m7Gppp5'N). Vaccinia virus capping enzyme can cap the RNA in correct direction within one hour when present at a suitable concentration of capping buffer, guanosine triphosphate (GTP), S-adenosylmethionine (SAM), etc.
This product is produced by GMP process requirements and provided in a liquid form, used for in vivo/in vitro pre-translation mRNA capping reaction or mRNA 5'end labeling reaction.

Features

Isolated from a recombinant source
Tested for the absence of endonucleases, exonucleases, RNases

Kit Components

Components No.	Name	10615ES84 (2,000 U)	10615ES92 (10,000 U)	10615ES96 (100,000 U)	10615ES99 (5 MU)
10615	mRNA Vaccinia Capping Enzyme (10 U/μL)	200 μL	1 mL	10 mL	500 mL

Shipping

mRNA Vaccinia Capping Enzyme GMP-grade products are shipped with dry ice.

Source

Recombinant E. coli with vaccinia virus capping enzyme gene

Storage Buffer	20 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM DTT, 0.1 mM EDTA, 0.1% Triton X-100, 50% glycerin
Storage	Stored at -15℃ ~ -25℃ for one year.

Application	Capping mRNA before translation assays/in vitro translation Labeling 5´ end of mRNA

The capping efficiency almost reached 100% by the vaccinia capping system from YEASEN. Nucleic acid mass spectrometry before the cap reaction was shown in Figure 1A. Nucleic acid mass spectrometry after the cap reaction was shown in Figure 1B.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHN20800035-1	10615ES92	10 KU	$1445		Add to Cart
BHN20800035-2	10615ES84	2 KU	$365		Add to Cart

mRNA Vaccinia Capping Enzyme (10 U/uL)

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