Hifair™ Precision sgRNA Synthesis Kit

Mfr.No.

11355ES60, 11355ES50, 11355ES25

Description

CRISPR/Cas9 is a technique in which RNA directs Cas nucleases to perform specific DNA modifications on targeted genes, resulting in an acquired immune defense mechanism evolved by bacteria and archaea in response to attacks by bacteriophages and exogenous plasmids. In mode biotechnology research, this system guides the nuclease Cas9 protein to cut double-stranded DNA at the target site paired with sgRNA by artificially optimized sgRNA (Single Guide RNA), causing DNA breaks. Due to the repair of DNA by non-homologous terminal repair mechanism or homologous recombination mechanism in organisms, gene shifting, mutation, replacement or deletion occur, resulting in loss of gene function.

The Hifair™ Precision sgRNA Synthesis Kit uses a T7 RNA polymerase to efficiently transcribe spCas9 sgRNA. This kit contains a specific sequence that is recognized by the Cas9 protein and acts as a scaffold, and part of this sequence can partially overlap with the user-designed specific sequence of interest. After annealing, complementary strands are formed, which are filled by DNA polymerase. The resulting dsDNA template for transcription is generated. Using the reagents provided and user-designed specific DNA sequences, this kit can obtain 20-100 μg of functional sgRNA in 4 hours in a single tube reaction.

Kit Components

No.	Name	11355ES25 (25 T)	11355ES50 (50 T)
11355-A	10×sgRNA Enzyme Mix	50 μL	100 μL
11355-B	10×sgRNA Reaction Buffer	50 μL	100 μL
11355-C	2×Canace Enzyme Mix	312.5 μL	625 μL
11355-D	Scaffold Template	31.25 μL	62.5 μL
11355-E	NTPs（25mM each）	200 μL	400 μL
11355-F	Control sgRNA Oligo（10μM）	10 μL	20 μL

Storage	-25℃ ~ -15℃ storage, valid for 2 years.

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Combination SKU	Supplier No.	Size	Your price
BHN20800025-1	11355ES60	100 T	$1055	Add to Cart
BHN20800025-2	11355ES50	50 T	$595	Add to Cart
BHN20800025-3	11355ES25	25 T	$325	Add to Cart

Hifair™ Precision sgRNA Synthesis Kit

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