The kit is designed for preparation of high quality total RNA from a wide range of fungal species. Fungal samples are lysed and homogenized, RNA in the whole homogeneity is then selectively absorbed on spin column and other impurities are washed away. Total RNA is eluted from the membrane in the presence of RNase-Free Water in the final step. 3-5?g total RNA can be purified from 30mg filamentous fungi using this kit. Purified RNA is ready for most downstream applications such as RT-PCR, Northe Blotting, Poly A+ purification, nuclease protection and in vitro translation.
Equipment Required
Microcentrifuge
Pipettes
Features
Fast. Using a rapid spin-column format, the entire procedure takes approximately 20 minutes.
High quality of RNA. OD260/280 of purified RNA is generally >1.8.
Intact RNA: NO RNA degradation and integrity maintained.
Storage
Kit components are stable for 12 months after receipt when stored at 4ºC.