Creatinine Serum Detection Kit

Mfr.No.	SKT-217
Alternative Names	N-Carbamimidoyl-N-methylglycine Detection Kit, Methylguanidoacetic acid Detection Kit
Categories	Detection Kits
Description	Colorimetric measurement of creatinine
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Gross Weight (kg)	2
Net Weight (g)	300
Reactivity	Rabbit, Human, Mouse, Sheep, Rat
Scientific Background	Creatinine (2-amino-1-methyl-5H-imadazol-4-one) is a metabolite of phosphocreatine (p-creatine), a molecule used as a store for high-energy phosphate that can be utilized by tissues for the production of ATP (1). Creatine either comes from the diet or synthesized from the amino acids arginine, glycine, and methionine. This occurs in the kidneys and liver, although other organ systems may be involved and species-specific differences may exist (2). Creatine and p-creatine are converted non-enzymatically to the metabolite creatinine, which diffuses into the blood and is excreted by the kidneys. In vivo , this conversion appears to be irreversible and in vitro it is favored by higher temperatures and lower pH2. Creatinine forms spontaneously from p-creatine (3). Under normal conditions, its formation occurs at a rate that is relatively constant and as intra- individual variation is <15% from day to day, creatinine is a useful tool for normalizing the levels of other molecules found in urine. Additionally altered creatinine levels may be associated with other conditions that result in decreased renal blood flow such as diabetes and cardiovascular disease (4-6).
Size	2 Plates ; 100 µl ; 6 ml ; 20 ml
Adr Code	Non-hazardous
Assay Overview	The Creatinine Serum Detection Kit is designed to quantitatively measure creatinine present in serum samples. A creatinine standard, calibrated to a NIST creatinine standard, is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or samples are pipetted into a clear microtiter plate. An assay diluent is added to all standards, controls and samples. The color generating reaction is initiated with the StressXpress® Creatinine Reagent, which is pipetted into each well. The assay utilizes a kinetic absorbance method to overcome interference by colored compounds in serum. The absorbance of the colored product is read after 1 minute in a microtiter plate reader capable of measuring 490nm wavelength. At 30 minutes the optical density is read again. The concentration of creatinine is calculated using the delta of the optical density readings at 30 and 1 minute compared to the curve generated from the standards, or by using the Excel worksheet available for free download at our web site. The Jaffe reaction used in this kit has been modified to read creatinine levels in serum,8.
Assay Range	0.5 - 4 mg/dl
Assay Type	Direct Quantitative Assay
Assay Type Abbreviation	Direct
Component Number	SKC-217A ; SKC-217B ; SKC-217C ; SKC-217D
Country Of Origin	Canada
Detection Method	Colorimetric Assay
Item	Clear 96 well Half Area Plates ; Creatinine Standard ; Assay Diluent ; StressXpress® Creatinine Reagent
Number Of Samples	91 samples in duplicate
Platform	Microplate
Precision	Intra Assay Precision: Three human serum samples were run in replicates of 20 in an assay. The mean and precision of the calculated creatinine concentrations were: Sample 1- 0.99 mg/dL, 7.9% CV Sample 2- 1.50 mg/dL, 6.3% CV Sample 3- 3.82 mg/dL, 4.5% CV Inter Assay Precision: Three human serum samples were run in duplicates in 19 assays run over two years by four operators. The mean and precision of the calculated creatinine concentrations were: Sample 1- 0.91 mg/dL, 9.6% CV Sample 2- 1.26 mg/dL, 7.3% CV Sample 3- 3.51 mg/dL, 8.0% CV
Sensitivity	0.081 mg/dl
Species Reactivity Abbreviations	Hu ; Ms ; Rb ; Rt ; Sh
Species Reactivity Medium	Human ; Mouse ; Rabbit ; Rat ; Sheep
Trademark	StressXpress®

Target	Creatinine Serum
Tarriff Code	3822.00.1090
Unspsc Code	12352203

Shipping Temperature	Blue Ice
Storage Temperature	4ºC
Stock Product	Y
Utility	Colorimetric assay used to measure creatinine in samples.

Reference	1. Wallimann, T. et al., Biochem. J., 2000, 281, 21-40. 2. Wyss, M. and Kaddurah-Daouk, R., Physiol. Rev., 2000, 80, 1107-1213. 3. Raja Iyengar, M. et al., J. Biol. Chem, 1985, 260, 7562-7567. 4. Manjunath, G. et al., Postgrad. Med. 2001, 110, 55-62. 5. Gross, J.L. et al., Diabetes Care, 2005, 28, 164-176. 6. Anavekar, N.S. et al., New Engl. J. Med., 2004, 351, 1285-1295.

Other Resources 1	Kit Booklet
Other Resources 2	MSDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHE11900035-1	SKT-217-192	2 X 96 well	$248		Add to Cart

Creatinine Serum Detection Kit

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