DMEM (Dulbecco’s Modified Eagle’s Medium) contains four-fold concentrations of the amino acids and vitamins as present in BME (Basal Medium Eagle). This culture medium, enriched with 4.5 g/L glucose, supports the proliferation of a wide variety of normal and transformed cells, such as primary cultures of mouse and chicken cells growing adherently.
The Buffering capacity ranges from pH = 7.2 to 7.6. Each lot of DMEM (CLS designation MG-30 has been tested for sterility and absence of Mycoplasma and Bacteria. The manufacturing process and documentation are performed according to ISO9001:2008 based on GLP under strict quality control.
Maintenance
Keep refrigerated at +2°C to +8°C in the dark. Freezing as well as warming up to +37°C minimize the quality of the product.
Do not heat the medium to more than 37°C or use uncontrollable sources of heat (e.g. microwave appliances).
If only a part of the medium is to be used, remove this amount from the bottle and warm it up inside a separate device.
Short Description
DMEM High Glucose, basic supplemented with 4.5 g/l glucose and L-glutamine Addition of serum or other supplementation required