FRTL Cell Growth medium is based on Coon’s modification of Ham’s F12 und supplemented with hormones, L-Glutamine and FBS. Together with the TSH stock solution coming with the medium, it is ideally suited for the propagation of FRTL and FRTL-5 cells. Directly before usage, add the respective amount of TSH. Each aliquot provides enough TSH for preparing 100 ml complete FRTL Cell Growth Medium with a final TSH concentration of 16.5 ng/ml.