The Calciumfectin™ Mammalian Transfection Reagent Kit provides high-quality reagents suitable for both transient and stable transfections of mammalian cells.
Kit Contents
6 ml 2 M Calcium Solution 50 ml 2X HEPES-Buffered Saline (HBS) 70 ml Sterile H2O
Notes
This product is distributed for laboratory research only. Caution: Not for diagnostic use .
Quantity
Kit (100 transfections)
Shipping Condition
Ice packs
Storage Condition
4C
Storage
Store HBS buffer at -20ºC; all other components at 4ºC.
Reference
Wong, Let al. "Cisplatin-resistant cancer cells are sensitive to Aurora kinase A inhibition by alisertib." Molecular Oncology 11 (8):981–995 (2017).DOI: 10.1002/1878-0261.12066. Application: Transfection.
He, Zet al. "MiR-143-3p functions as a tumor suppressor by regulating cell proliferation, invasion and epithelial–mesenchymal transition by targeting QKI-5 in esophageal squamous cell carcinoma." Molecular Cancer 15 (1):51 (2017).DOI: 10.1186/s12943-016-0533-3. Application: Transfection.
Chen, Zet al. "Gastric tumour-derived ANGPT2 regulation by DARPP-32 promotes angiogenesis.." Gut 6:925-934 (2016).DOI: 10.1136/gutjnl-2014-308416. Application: generate recombinant adenoviruses.
Shoumin, Zet al. "Helicobacter pylori-induced cell death is counteracted by NF-κB-mediated transcription of DARPP-32." Gut : (2016).DOI: doi:10.1136/gutjnl-2016-312141. Application: generate adenoviruses.
Hong, Jet al. "ABL regulation by AXL promotes cisplatin resistance in esophageal cancer." Cancer Res. 73(1):331-340 (2013).DOI: 10.1158/0008-5472.CAN-12-3151.
Katsha, Aet al. "Aurora kinase A promotes inflammation and tumorigenesis in mice and human gastric neoplasia." Gastroenterology 145(6):1312-22.e1-8 (2013).DOI: 10.1053/j.gastro.2013.08.050.PubMed: 23993973.
Hong, Jet al. "Regulation of ERBB2 Receptor by t-DARPP Mediates Trastuzumab Resistance in Human Esophageal Adenocarcinoma." Cancer Res. 72(17):4504-4514 (2012).DOI: 10.1158/0008-5472.CAN-12-1119.
Zhu, Set al. "DARPP-32 increases interactions between epidermal growth factor receptor and ERBB3 to promote tumor resistance to gefitinib. DARPP-32 increases interactions between epidermal growth factor receptor and ERBB3 to promote tumor resistance to gefitinib." Gastroenterology 141(5):1738-48.e1-2. (2011).DOI: 10.1053/j.gastro.2011.06.070.
Katsha, Aet al. "Activation of EIF4E by Aurora Kinase A Depicts a Novel Druggable Axis in Everolimus-Resistant Cancer Cells." Clinical Cancer Research : (0).DOI: 10.1158/1078-0432.CCR-16-2141. Application: Transfection.