COVID-19 Spike Protein Accquant ELISA Kit

Mfr.No.

AQ-U3128

Sensitivity

390fg/ml

Detection Range

0.78-50pg/ml

Reactivity

Coronavirus

Assay Duration

5 hours

Assay Type

Sandwich

Alternative Names

Spike glycoprotein ELISA Kit, S glycoprotein ELISA Kit, E2 ELISA Kit, Peplomer protein ELISA Kit, Spike protein S1 ELISA Kit, Spike protein S2 ELISA Kit, Spike protein S2' ELISA Kit, S ELISA Kit

Detection Method

Double Antibody

Recovery

Add a certain amount of 2019-nCoV (S) into the sample. Calculate the recovery by comparing the measured value with the expected amount of 2019-nCoV (S) in the sample.

Sample Type	Recovery Range(%)	Average(%)
serum(n=10)	88-104	96
EDTA plasma(n=10)	92-100	95
Heparin plasma(n=10)	90-105	95

Linearity

Dilute the sample with a certain amount of 2019-nCoV (S) at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type	1:2	1:4	1:8
serum(n=10)	92-100%	88-105%	90-98%
EDTA plasma(n=10)	86-99%	90-101%	87-96%
Heparin plasma(n=10)	84-100%	83-94%	82-94%

Specificity

Specifically binds with 2019-nCoV (S) , no obvious cross reaction with other analogues.

Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.) Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(pg/ml)	OD-1	OD-2	Average
0	0.158	0.166	0.161
0.781	0.206	0.216	0.21
1.562	0.233	0.245	0.238
3.125	0.314	0.33	0.32
6.25	0.46	0.483	0.469
12.5	0.719	0.756	0.734
25	1.21	1.272	1.235
50	2.124	2.232	2.167

Assay Procedure Summary

Step 1:
Take out the required plate wells, add 50ul Cap/Det Ab into each well, then add 50ul Standard or Sample into individual well. (When adding standard or sample, the disposable tip lightly touches the liquid level. Change the disposable tips for different samples and standards.) Gently tap the plate for 10s to ensure thorough mixing then static incubate for 60 minutes at 37°C.

Washing:
Wash the plate twice without immersion.

Step 2:
Add 100ul HRP-Streptavidin (orange) into each well, seal the plate and static incubate for 30 minutes at 37°C.

Washing:
Wash the plate five times without immersion.

Step 3:
Add 90ul TMB substrate solution, seal the plate and static incubate for 10-20 minutes at 37°C. (Accurate TMB visualization control is required.)

Step 4:
Add 50ul stop solution. Read at 450nm immediately and calculate.

Precision

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/ml)	1.61	6.31	25.17	1.55	6.52	24.61
Standard deviation	0.07	0.36	1.39	0.08	0.31	1.3
CV(%)	4.49	5.72	5.54	4.88	4.68	5.27

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Notes

For Research Use Only

Usage Notes

Microplate reader (wavelength: 450nm)
37°C incubator (CO2 incubator for cell culture is not recommenced.)
Automated plate washer or multi-channel pipette/5ml pipettor (for manual washing purpose)
Precision single (0.5-10uL, 5-50uL, 20-200uL, 200-1000uL) and multi-channel pipette with disposable tips(Calibration is required before use.)
Sterile tubes and Eppendorf tubes with disposable tips
Absorbent paper and loading slot
Deionized or distilled water

Protocol

AQ-U3128.pdf
MSDS

UniProt ID	P0DTC2

Applications	ELISA
Applications Notes	COVID-19 Spike Protein Accquant ELISA Kit allows for the in vitro quantitative determination of COVID-19 Sipke ELISA Kit concentrations in serum, plasma, tissue homogenates and other biological fluids.

Storage	2-8°C(Sealed), Don't cryopreserve.

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SKU	Supplier No.	Size	Your price	Quantity
BHE10807647	AQ-U3128	96T	$350		Add to Cart

COVID-19 Spike Protein (RBD) Accquant ELISA Kit

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