This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Host
Mouse
Isotype
IgG
Quantity
100 µg
Recombinant
FALSE
Regulatory
RUO
Shipping Condition
Ice Packs
Buffer
PBS, pH 7.4 with 0.05% sodium azide.
Concentration
100µg/100ul
Format
Liquid
Storage
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition
-20C
Applications
WB, IF
Description
It is very useful as a loading control for quantitative western blotting. The antibody recognizes a 37kDa band in a Western Blot analysis., WB ; IF
Reference
Bastien, B et al. "IL-1 Gene Deletion Protects Oligodendrocytes after Spinal Cord Injury through Upregulation of the Survival Factor Tox3 ." J. Neurosci. 30:10715–10730 (2015). DOI: 10.1523/JNEUROSCI.0498-15.2015. Application: WB Control.
Belova, S et al. "Paradoxical effect of IKKβ inhibition on the expression of E3 ubiquitin ligases and unloading‐induced skeletal muscle atrophy." Physiological Reports e13291: (2017). DOI: 10.14814/phy2.13291. PubMed: 28839114 . Application: Western Blot.
Bessière, L.B. et al. "A Hot-spot of In-frame Duplications Activates the Oncoprotein AKT1 in Juvenile Granulosa Cell Tumors." EBioMedicine 5:421-431 (2015). DOI: 10.1016/j.ebiom.2015.03.002. Application: WB.
Bryand, KF et al. "Identification of a divalent metal cation binding site in herpes simplex virus 1 (HSV-1) ICP8 required for HSV replication." J Virol 86 (12):6825-6834 (2012). DOI: 10.1128/JVI.00374-12. Application: Immunoblot.
Chahi, A et al. "Nephrin Suppresses Hippo Signaling through the Adaptor Proteins Nck and WTIP." The Journal of Biological Chemistry (291):12799-12808 (2016). DOI: 10.1074/jbc.M116.724245. PubMed: 27033705. Application: Western Blot.
Cicchetti, F et al. "Mutant huntingtin is present in neuronal grafts in huntington disease patients." Ann. Neurol. 1:31-42 (2014). PubMed: 24798518.
Cicchetti, F et al. "Mutant huntingtin is present in neuronal grafts in Huntington's disease patients." Ann. Neurol. : (2014). PubMed: 24798518.
Clouthier, D.L.C. et al. "Requisite Role for Nck Adaptors in Cardiovascular Development, Endothelial-to-Mesenchymal Transition, and Directed Cell Migration." Molecular and cellular biology 9:1573-1587 (2015). DOI: 10.1128/MCB.00072-15. Application: WB.
Evans, RP et al. "Aurora A kinase RNAi and small molecule inhibition of Aurora kinases with VE-465 induce apoptotic death in multiple myeloma cells." Leuk Lymphoma 49 (3):559-569 (2008). DOI: 10.1080/10428190701824544. Application: Immunoblot.
Evans, RP et al. "Expression, adverse prognostic significance and therapeutic small molecule inhibition of Polo-like kinase 1 in multiple myeloma." Leuk Res 35 (12):1637-1640 (2011). DOI: 10.1016/j.leukres.2011.07.016. PubMed: 21816470. Application: Immunoblot.
Evans, RP et al. "The selective Aurora B kinase inhibitor AZD1152 is a potential new treatment for multiple myeloma." Br J Haematol 140 (3):295-302 (2008). PubMed: 18076711. Application: Immunoblot.
Gerwing, M et al. "Cabazitaxel overcomes cisplatin resistance in germ cell tumour cells." Journal of Cancer Research and Clinical Oncology 9(142):1979-1994 (2016). DOI: 10.1007/s00432-016-2204-6. PubMed: 27424191. Application: Western Blot.
Kang, HT et al. "Chemical screening identifies ROCK as a target for recovering mitochondrial function in Hutchinson-Gilford progeria syndrome." Aging Cell 3:541-550 (2017). DOI: 10.1111/acel.12584. PubMed: 28317242 . Application: Western Blot.
Knipe, D et al. "Foreign DNA Surveillance Protein." http://www.freepatentsonline.com/y2016/0076041.html : (0). DOI: 14/888853. Application: Western Blot.
New, LA et al. " Direct Regulation of Nephrin Tyrosine Phosphorylation by Nck Adaptor Proteins." J Biol Chem 288 (3):1500-1510 (2013). DOI: 10.1074/jbc.M112.439463. Application: Western Blot.
Shintani-ishida, K et al. "MDMA induces cardiac contractile dysfunction through autophagy upregulation and lysosome ." Biochim. Biophys. Acta, Mol. Basis. Dis. 5:691-700 (2014). PubMed: 24491919.
Shintani-ishida, K et al. "MDMA induces cardiac contractile dysfunction through autophagy upregulation and lysosome destabilization in rats." Biochim. Biophys. Acta 1842:691-700 (2014). PubMed: 24491919.
Smith, GS et al. "Nucleus-localized 21.5-kDa myelin basic protein promotes oligodendrocyte proliferation and enhances neurite outgrowth in coculture, unlike the plasma membrane-associated 18.5-kDa isoform." J Neurosci Res. 91 (3):349-362 (2013). DOI: 10.1002/jnr.23166. PubMed: 23184356. Application: Western Blot.
Vilchinskaya, N et al. "Rapid decline in MyHC I(β) mRNA expression in rat soleus during hindlimb unloading is associated with AMPK dephosphorylation." The Journal of physiology 23:7123-7134 (2017). DOI: 10.1113/JP275184. PubMed: 28975644 . Application: Western Blot.
Yoshida, K.Y. et al. "Mitochondrial m-calpain opens the mitochondrial permeability transition pore in ischemia–reperfusion." Int. J. Cardiol. Heart Vessels N/A:26-32 (2015). DOI: 10.1016/j.ijcard.2015.06.010.
Reviews of Anti-GAPDH Loading Control Monoclonal Antibody