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Anti-Beta-Actin Monoclonal Loading Control Antibody

BHA10915550
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Contact us to order
Tel
+1 866.986.9598
Email
[email protected]
Get a Quote
Ask Merchant
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Buffer PBS, pH 7.4 with 0.05% sodium azide.
Concentration 100µg/100ul
Description Specificity: Band size at approximately 40kDA on western blot
Format Liquid
Purity Affinity Purified.
Storage This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Storage Condition -20C
Applications WB, ELISA
Description 1:1000-10,000 dilution (incubation for one hour at room temperature) for Western blot with ECL. In order to obtain best results in other assays (ELISA, etc.), please determine optimal working dilution by titration test., WB ; ELISA
Reference
  • Balboula, AZ et al. " Cathepsin B activity has a crucial role in the developmental competence of bovine cumulus–oocyte complexes exposed to heat shock during in vitro maturation." Reproduction 146 (4):407-417 (2013). DOI: 10.1530/REP-13-0179. PubMed: 23898216. Application: Western Blot.
  • Balboula, AZ et al. "Cathepsin B activity is related to the quality of bovine cumulus oocyte complexes and its inhibition can improve their developmental competence." Mol Reprod Dev 77 (5):439-448 (2010). DOI: 10.1002/mrd.21164. Application: Western Blot.
  • Byongsun, L et al. "Emerin suppresses Notch signaling by restricting the Notch intracellular domain to the nuclear membrane." Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 2:303-313 (2017). DOI: 10.1016/j.bbamcr.2016.11.013. Application: Loading Control Antibody.
  • Carpino, N et al. "The Sts proteins target tyrosine phosphorylated, ubiquitinated proteins within TCR signaling pathways." Mol Immunol 46 (16):32241-3231 (2009). DOI: 10.1016/j.molimm.2009.08.015. PubMed: 19733910. Application: Immunoblot.
  • Chen, G et al. "The tumor suppressor ING3 is degraded by SCFSkp2-mediated ubiquitin–proteasome system." Oncogene 29 (10):1498-1508 (2010). DOI: 10.1038/onc.2009.424. Application: Western Blot.
  • Cho, KA et al. "Hypoxia Affected SDF-1α-CXCR4 Interaction between Bone Marrow Stem Cells and Osteoblasts via Osteoclast Modulation." Acta Haematol 123 (1):43-47 (2010). DOI: 10.1159/000262750. PubMed: 19955710. Application: Western Blot.
  • Cho, KA et al. "IL-33 induces Th17-mediated airway inflammation via mast cells in ovalbumin-challenged mice." Am J Physiol Lung Cell Mol Physiol 302 (4):L429-L420 (2012). DOI: 10.1152/ajplung.00252.2011. Application: Western Blot.
  • Cicchetti, F.C. et al. "Cerebrovascular and blood–brain barrier impairments in Huntington's disease: Potential implications for its pathophysiology." Ann Neurol 2:160-177 (2015). DOI: 10.1002/ana.24406.
  • Fukada, M et al. "TBC1D12 is a novel Rab11-binding protein that modulates neurite outgrowth of PC12 cells." PLoS One 4:e0174883 (2017). DOI: 10.1371/journal.pone.0174883. PubMed: 28384198. Application: Western Blot.
  • Hashimoto, H et al. "Vitronectin promotes the progress of the initial differentiation stage in cerebellar granule cells." Molecular and Cellular Neuroscience :76-85 (2015). DOI: 10.1016/j.mcn.2015.11.013. Application: WB.
  • Hashimoto, K et al. "Vitronectin Regulates the Fibrinolytic System during the Repair of Cerebral Cortex in Stab-Wounded Mice." Journal of Neurotrauma 22:3183-3191 (2017). DOI: 10.1089/neu.2017.5008. PubMed: 28683586 . Application: Western Blot.
  • Inoue, C et al. "SMARCD1 regulates senescence-associated lipid accumulation in hepatocytes." NPJ aging and mechanisms of disease 1:11 (2017). DOI: 10.1038/s41514-017-0011-1. Application: Western Blot.
  • Ishibashi, K et al. "Atg16L1, an essential factor for canonical autophagy, participates in hormone secretion from PC12 cells independently of autophagic activit." Mol Biol Cell 23 (16):3193-3202 (2012). DOI: 10.1091/mbc.E12-01-0010. Application: Immunoblot.
  • Jang, HJ. "Establishment and characterization of EBV positive B-lymphoblastoid cell lines derived from cancers." Thesis from Seoul National University : (2017). Application: Western Blot.
  • Kan, E.T. et al. "Structure-Function Analyses of the Small GTPase Rab35 and Its Effector Protein Centaurin-β2/ACAP2 during Neurite Outgrowth of PC12 Cells." The Journal of Biological Chemistry :9064-9074 (2015). DOI: 10.1074/jbc.M114.611301.
  • Ki, MK et al. "Human antibodies targeting the C-type lectin-like domain of the tumor endothelial cell marker clec14a regulate angiogenic properties in vitro." Oncogene 32:5449-57 (2013). PubMed: 23644659.
  • Kim, BK et al. "Hairless down-regulates expression of Msx2 and its related target genes in hair follicles." J. Dermatol. Sci. 71:203-9 (2013). PubMed: 23702391. Application: Western Blot .
  • Kim, BK et al. "Hairless plays a role in formation of inner root sheath via regulation of Dlx3 gene." J. Biol. Chem. 16681-8:287 (2012). PubMed: 22442153. Application: Western Blot.
  • Kurisu, K et al. "p16 Gene Transfer Induces Centrosome Amplification and Abnormal Nucleation Associated with Survivin Downregulation in Glioma Cells." Pathobiology 1:1-8 (2015). DOI: 10.1159/000368196.
  • Lee, YB et al. "Microcontact printing of polydopamine on thermally expandable hydrogels for controlled cell adhesion and delivery of geometrically defined microtissues." Acta Biomater. :75-87 (2017). DOI: 10.1016/j.actbio.2017.07.040. Application: Western Blot.
  • Lee, YB et al. "Polydopamine-mediated immobilization of multiple bioactive molecules for the development of functional vascular graft materials." Biomaterials 33 (33):8343-8352 (2012). DOI: 10.1016/j.biomaterials.2012.08.011. Application: Western Blot.
  • Lussier, MP et al. "Casein kinase 2 phosphorylates GluA1 and regulates its surface expression." Eur. J. Neurosci. 39:1148-58 (2014). PubMed: 24712994.
  • Marubashi, S.M. et al. "RUTBC1 Functions as a GTPase-acitvating Protein for Rab32/38 and Regulates Melanogenic Enzyme Trafficking in Melanocytes." Journal of Biological Chemistry : (2015). DOI: 10.1074/jbc.M115.684043. Application: WB.
  • Matsui, T et al. "The Rab Interacting Lysosomal Protein (RILP) Homology Domain Functions as a Novel Effector Domain for Small GTPase Rab36." J Biol Chem 287 (34):28619-28631 (2012). DOI: 10.1074/jbc.M112.370544. Application: Western Blot.
  • Matsui, T.M. et al. "Theaflavins enhance intestinal barrier of Caco-2 Cell monolayers through the expression of AMP-activated protein kinase-mediated Occludin, Claudin-1, and ZO-1." Bioscience, Biotechnology, and Biochemistry  1: (2015). DOI: 10.1080/09168451.2014.951027.
  • Matsui, TS et al. "Actin stress fibers are at a tipping point between conventional shortening and rapid disassembly at physiological levels of MgATP." Biochem Biophys Res Commun. 395 (3):301-306 (2010). DOI: 10.1016/j.bbrc.2010.03.150. PubMed: 20353757. Application: Immunostaining.
  • Matsui, TS et al. "Non-muscle myosin II induces disassembly of actin stress fibres independently of myosin light chain dephosphorylation." Interface Focus 1:754-766 (2011). DOI: 10.1098/rsfs.2011.0031. PubMed: 23050080. Application: Immunostaining.
  • Megumi, A.M. et al. "Small GTPase Rab2B and Its Specific Binding Protein Golgi-associated Rab2B Interactor-like 4 (GARI-L4) Regulate Golgi Morphology." The Journal of Biological Chemistry :22250-22261 (2015). DOI: 10.1074/jbc.M115.669242.
  • Miller, S et al. "A rapid and sensitive reporter gene that uses green fluorescent protein expression to detect chemicals with estrogenic activity." Toxicol Sci 55(1):69-77 (2000). PubMed: 10788561.
  • Morisugi, T et al. "Mechanical stretch enhances NF-kappaB-dependent gene expression and poly(ADP-ribose) synthesis in synovial cells." J Biochem 147 (5):633-644 (2010). DOI: 10.1093/jb/mvp210. Application: Western Blot.
  • Nishizono, A.N. et al. "Efficacy of favipiravir (T-705) in rabies post-exposure prophylaxis." J Infect Dis : (2015). DOI: 10.1093/infdis/jiv586.
  • Ohbayashi, N et al. "The Rab21-GEF activity of Varp, but not its Rab32, 38 effector function, is required for dendrite formation in melanocytes." Mol Biol Cell 23:669-78 (2012). PubMed: 22171327.
  • Ohkubo, O et al. "Leptin activates chicken growth hormone promoter without chicken STAT3 in vitro." Comp Biochem Physiol B Biochem Mol Biol. :46-52. (2015). PubMed: 26403688.
  • Ohta, M et al. "Novel dominant-negative mutant of GATA3 in HDR syndrome." J. Mol. Med. 89:43-50 (2011). PubMed: 21120445.
  • Ryu, CH et al. " Valproic acid downregulates the expression of MGMT and sensitizes temozolomide-resistant glioma cells." J Biomed Biotechnol 2012:987495 (2012). DOI: 10.1155/2012/987495. PubMed: 22701311. Application: Western Blot.
  • Sanz-Clemente, A et al. "Casein kinase 2 regulates the NR2 subunit composition of synaptic NMDA receptors." Neuron 67:984-96 (2010). PubMed: 20869595.
  • Sasai, M et al. "Potential contribution of the hepcidin-macrophage axis to plaque vulnerability in acute myocardial infarction in human." Int. J. Cardiol. :114-121 (2017). DOI: 10.1016/j.ijcard.2016.11.147. PubMed: 27855289 . Application: Western Blot.
  • Sciacca, G et al. "Mutant huntingtin protein expression and blood–spinal cord barrier dysfunction in huntington disease." Annals of Neurology 6:981-994 (2017). DOI: 10.1002/ana.25107. PubMed: 29171910. Application: Western Blot.
  • Shimamura, Y et al. "Inhibitory effects of Japanese horseradish (Wasabia japonica) on the formation and genotoxicity of a potent carcinogen, acrylamide." J. Sci. Food Agric. 8:2419-2425 (2017). DOI: 10.1002/jsfa.8055. PubMed: 27670634. Application: Western Blot.
  • Shin, H et al. "Microcontact printing of polydopamine on thermally expandable hydrogels for controlled cell adhesion and delivery of geometrically defined microtissues." Acta Biomater. :75-87 (2017). DOI: 10.1016/j.actbio.2017.07.040. PubMed: 28760620. Application: Western Blot.
  • Shin, HN et al. "Stromal cell-derived factor-1α and macrophage migration-inhibitory factor induce metastatic behavior in CXCR4-expressing colon cancer cells." Int. J. Mol. Med. 30:1537-43 (2012). PubMed: 23023114.
  • St-Amour, I et al. "Impact of intravenous immunoglobulin on the dopaminergic system and immune response in the acute MPTP mouse model of Parkinson's disease." J Neuroinflammation 9:324 (2012). DOI: 10.1186/1742-2094-9-234. Application: Immunoblot.
  • Sudo, H et al. "Caspase 3 as a therapeutic target for regulation of intervertebral disc degeneration in rabbits." Arthritis Rheum 63(6):1648-57 (2011). DOI: 10.1002/art.30251. PubMed: 21305515.
  • Sudo, H et al. "Regulation of apoptosis in nucleus pulposus cells by optimized exogenous Bcl-2 overexpression." J. Orthop. Res. 28:1608-13 (2010). PubMed: 20589931.
  • Sun, W et al. "Interleukin-1β promotes hypoxia-induced apoptosis of glioblastoma cells by inhibiting hypoxia-inducible factor-1 mediated adrenomedullin production." Cell Death Dis 5:e1020 (2014). PubMed: 24457964.
  • Suzuki, H et al. "A spontaneous mutation of the Wwox gene and audiogenic seizures in rats with lethal dwarfism and epilepsy." Genes Brain Behav. 8:650-60 (2009). PubMed: 19500159.
  • Taira, N.T. et al. "Enhanced cytotoxicity of nitidine against camptothecin-resistant A549 cells." Traditional & Kampo Medicine 8:N/A (2015). DOI: 10.1002/tkm2.1036. Application: WB.
  • Vandal, M et al. "Reduction in DHA transport to the brain of mice expressing human APOE4 compared to APOE2." J. Neurochem. 129:516-26 (2014). PubMed: 24345162.
  • Yamada, K et al. " Addition of a single N-glycan to street rabies virus glycoprotein enhances virus production." J Gen Virol 94 (Pt 2):270-5 (2013). PubMed: 23114627. Application: Western Blot.
  • Yamada, K et al. "Characterization of street rabies virus variants with an additional N-glycan at position 247 in the glycoprotein." Arch. Virol. 159:207-16 (2013). PubMed: 23921623. Application: Western Blot.
  • Yamada, K et al. "Serial passage of a street rabies virus in mouse neuroblastoma cells resulted in attenuation: potential role of the additional N-glycosylation of a viral glycoprotein in the reduced pathogenicity of street rabies virus." Virus Res 165 (1):34-45 (2012). DOI: 10.1016/j.virusres.2012.01.002. PubMed: 22248643. Application: Western Blot.
  • Yamanaka, K et al. "Effects of downregulating DNA methyltransferase 1 transcript by RNA interference on DNA methylation status of the satellite I region and in vitro development of bovine somatic cell nuclear transfer embryos." J Reprod Dev. 57 (3):393-402 (2011). PubMed: 21343670. Application: Western Blot.
  • Yamanaka, K et al. "Gene silencing of DNA methyltransferases by RNA interference in bovine fibroblast cells." J Reprod Dev. 56 (1):60-67 (2010). PubMed: 19815986. Application: Western Blot.
  • Yamashiro, Y et al. "Ectopic coexpression of keratin 8 and 18 promotes invasion of transformed keratinocytes and is induced in patients with cutaneous squamous cell carcinoma." Biochem Biophys Res Commun 399 (3):365-372 (2010). DOI: 10.1016/j.bbrc.2010.07.077. PubMed: 20659422. Application: Immunoblot.
  • Yamashita, M et al. "Suppression of immunoglobulin production in human peripheral blood mononuclear cells by monocytes via secretion of heavy-chain ferritin." Immunobiology 219:149-57 (2014). PubMed: 24157279. Application: Western Blot.
  • Yatsu, A et al. "Syntaxin-3 is required for melanosomal localization of Tyrp1 in melanocytes." J Invest Dermatol 133:2237-46 (2013). PubMed: 23549422.
  • Yen, YK et al. "Western blotting by thin-film direct coating." Anal Chem 86:5164-70 (2014). PubMed: 24773468.
  • Yotsumoto, K et al. "5-Fluorouracil treatment alters the expression of intestinal transporters in rats." Biopharmaceutics & Drug Disposition 9:509-516 (2017). DOI: 10.1002/bdd.2102. PubMed: 28849584 . Application: Western Blot.
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BHA10915550 G043 100 ug $247.50
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